In vivo systemic chlorogenic acid therapy under diabetic conditions: Wound healing effects and cytotoxicity/genotoxicity profile
Autor: | Nihal Y. Gul, Mine Sibel Gurun, Zulfiye Gul, Ayse Topal, Ozge Turacozen, Sevda Inan, Betul Cam Etoz, Nilufer Cinkilic, Sedef Ziyanok, Sibel Tas, Musa Ozgur Ozyigit, Deniz Bagdas |
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Přispěvatelé: | Uludağ Üniversitesi/Tıp Fakültesi/Deney Hayvanları Yetiştirme ve Araştırma Merkezi., Uludağ Üniversitesi/Tıp Fakültesi/Fizyoloji Anabilim Dalı., Uludağ Üniversitesi/Tıp Fakültesi/Farmakoloji Anabilim Dalı., Uludağ Üniversitesi/Fen ve Edebiyat Fakültesi/Biyoloji Bölümü., Uludağ Üniversitesi/Veteriner Fakültesi/Patoloji Anabilim Dalı., Uludağ Üniversitesi/Veteriner Fakültesi/Cerrahi Anabilim Dalı., Bağdaş, Deniz, Etöz, Betül Cam, Gül, Zülfiye, Ziyanok, Sedef, İnan, Sevda, Turaçözen, Özge, Gül, Nihal, Topal, Ayşe B., Çinkılıç, Nilüfer, Taş, Sibel, Özyiğit, Musa Özgür, Gürün, Mine Sibel, AAR-6478-2021, AAF-9939-2020, AAG-8716-2019, E-3364-2018, ABE-6873-2020, AAH-4272-2021, AAH-2873-2021, AAH-5296-2021 |
Rok vydání: | 2014 |
Předmět: |
Blood Glucose
Male Antioxidant Diabetic wound healing Cytotoxicity medicine.medical_treatment Instant coffee Glucose blood level Pharmacology Toxicology medicine.disease_cause Biochemistry Animal tissue Antioxidants Rats Sprague-Dawley In vivo study chemistry.chemical_compound Diabetes mellitus Nitric-oxide Malondialdehyde Pathology Medicine Drug safety Sprague Dawley rat biology Experimental diabetes mellitus Malonaldehyde General Medicine Catalase Glutathione Hydroxyproline Rat model Prooxidant activities Chlorogenic Acid Drug mechanism Histopathology Food science & technology Nitric Oxide Article Streptozocin Carlina Oxide Lonicera Nitric oxide Diabetes Mellitus Experimental Dna-damage Superoxide dismutase Phenethyl ester Animalia Animals Animal model Animal experiment Caffeic acid Wound Healing Drug effects Rattus Animal business.industry Superoxide Dismutase Wound Nonhuman Rats Topical application Drug efficacy Oxidative Stress Glucose Metabolism chemistry biology.protein Rat Lipid Peroxidation Genotoxicity Wound healing business Reactive Oxygen Species Reactive oxygen metabolite Controlled study Oxidative stress Food Science DNA Damage |
Zdroj: | Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association. 81 |
ISSN: | 1873-6351 |
Popis: | Oxidative stress occurs following the impairment of pro-oxidant/antioxidant balance in chronic wounds and leads to harmful delays in healing progress. A fine balance between oxidative stress and endogenous antioxidant defense system may be beneficial for wound healing under redox control. This study tested the hypothesis that oxidative stress in wound area can be controlled with systemic antioxidant therapy and therefore wound healing can be accelerated. We used chlorogenic acid (CGA), a dietary antioxidant, in experimental diabetic wounds that are characterized by delayed healing. Additionally, we aimed to understand possible side effects of CGA on pivotal organs and bone marrow during therapy. Wounds were created on backs of streptozotocin-induced diabetic rats. CGA (50 mg/kg/day) was injected intraperitoneally. Animals were sacrificed on different days. Biochemical and histopathological examinations were performed. Side effects of chronic antioxidant treatment were tested. CGA accelerated wound healing, enhanced hydroxyproline content, decreased malondialdehyde/nitric oxide levels, elevated reduced-glutathione, and did not affect superoxide dismutase/catalase levels in wound bed. While CGA induced side effects such as cyto/genotoxicity, 15 days of treatment attenuated blood glucose levels. CGA decreased lipid peroxidation levels of main organs. This study provides a better understanding for antioxidant intake on diabetic wound repair and possible pro-oxidative effects. |
Databáze: | OpenAIRE |
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