Effect of the electro-acupuncture on senile plaques and its formation in APP+/PS1+ double transgenic mice
| Autor: | Kai-Hui Wu, Shu-Juan Zhu, Hua-Jun Sheng, Yiying Wang, Yan-Jun Wu, Jin Jin Xu, Cheng-Lin Tang, Qing-Hua Yang |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Genetically modified mouse
medicine.medical_specialty Amyloid β-protein AchE cholinesterase Senile plaques Stimulation Biochemistry Article Pathogenesis 03 medical and health sciences 0302 clinical medicine Western blot SOD superoxide dismutase Internal medicine mental disorders medicine Insulin-degrading enzyme Amyloid precursor protein Electro-acupuncture Transgenic mice IDE insulin degrading enzyme Ach aetylcholine Molecular Biology Genetics (clinical) EA electro-acupuncture amyloid precursor protein BACE1 Aβ amyloid β-protein medicine.diagnostic_test biology business.industry β－ site APP cleavage Cell Biology Alzheimer's disease APP β－ Endocrinology SPs senile plaques 030220 oncology & carcinogenesis biology.protein Immunohistochemistry ChAT aetylcholine tansferase AD Alzheimer's disease business IHC immunohistochemistry 030217 neurology & neurosurgery |
| Zdroj: | Genes & Diseases |
| ISSN: | 2352-3042 |
| DOI: | 10.1016/j.gendis.2018.06.002 |
| Popis: | Alzheimer's disease (AD) is a progressive neurodegenerative disease and its incidence will increase with age and is aggravating. The senile plaques (SPs) are one of three main pathological features in AD patients, which are formed by amyloid β-protein (Aβ) over-accumulation. β-amyloid precursor protein (APP), β-site APP cleavage (BACE1), and insulin degrading enzyme (IDE) proteins participate in the process of Aβ production and degradation. At present, the pathogenesis of AD is not yet clear and the current treatment methods can only relief the related symptoms of AD. The electro-acupuncture (EA) is a traditional Chinese medicine treatment combined the acupuncture and electrical stimulation and the treatment effect can also be controlled by transform the electrical frequency. Thus, in this experiment, we carried out behavioral test, immunohistochemistry (IHC), and Western Blot (WB) after different period treatments to the model mice by electro-acupuncturing “Baihui” and “Shenshu” acupoints in APP+/PS1+ double transgenic mice. It was found that the EA therapy can improve the ability of learning, memory and spatial exploration, and reduce the deposition of SPs in brain of AD model mice, and reduce the expressions of APP and BACE1, increase the expression of IDE protein. These results prompt that EA can effectively alleviate the pathological process of AD. We speculate that EA may play a comprehensive role in preventing the development of AD, considering the previous data. |
| Databáze: | OpenAIRE |
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