Effect of the electro-acupuncture on senile plaques and its formation in APP+/PS1+ double transgenic mice

Autor: Kai-Hui Wu, Shu-Juan Zhu, Hua-Jun Sheng, Yiying Wang, Yan-Jun Wu, Jin Jin Xu, Cheng-Lin Tang, Qing-Hua Yang
Rok vydání: 2019
Předmět:
Genetically modified mouse
medicine.medical_specialty
Amyloid β-protein
AchE
cholinesterase
Senile plaques
Stimulation
Biochemistry
Article
Pathogenesis
03 medical and health sciences
0302 clinical medicine
Western blot
SOD
superoxide dismutase
Internal medicine
mental disorders
medicine
Insulin-degrading enzyme
Amyloid precursor protein
Electro-acupuncture
Transgenic mice
IDE
insulin degrading enzyme
Ach
aetylcholine
Molecular Biology
Genetics (clinical)
EA
electro-acupuncture
amyloid precursor protein
BACE1
Aβ
amyloid β-protein
medicine.diagnostic_test
biology
business.industry
β－
site APP cleavage
Cell Biology
Alzheimer's disease
APP
β－
Endocrinology
SPs
senile plaques
030220 oncology & carcinogenesis
biology.protein
Immunohistochemistry
ChAT
aetylcholine tansferase
AD
Alzheimer's disease
business
IHC
immunohistochemistry
030217 neurology & neurosurgery
Zdroj: Genes & Diseases
ISSN: 2352-3042
DOI: 10.1016/j.gendis.2018.06.002
Popis: Alzheimer's disease (AD) is a progressive neurodegenerative disease and its incidence will increase with age and is aggravating. The senile plaques (SPs) are one of three main pathological features in AD patients, which are formed by amyloid β-protein (Aβ) over-accumulation. β-amyloid precursor protein (APP), β-site APP cleavage (BACE1), and insulin degrading enzyme (IDE) proteins participate in the process of Aβ production and degradation. At present, the pathogenesis of AD is not yet clear and the current treatment methods can only relief the related symptoms of AD. The electro-acupuncture (EA) is a traditional Chinese medicine treatment combined the acupuncture and electrical stimulation and the treatment effect can also be controlled by transform the electrical frequency. Thus, in this experiment, we carried out behavioral test, immunohistochemistry (IHC), and Western Blot (WB) after different period treatments to the model mice by electro-acupuncturing “Baihui” and “Shenshu” acupoints in APP+/PS1+ double transgenic mice. It was found that the EA therapy can improve the ability of learning, memory and spatial exploration, and reduce the deposition of SPs in brain of AD model mice, and reduce the expressions of APP and BACE1, increase the expression of IDE protein. These results prompt that EA can effectively alleviate the pathological process of AD. We speculate that EA may play a comprehensive role in preventing the development of AD, considering the previous data.
Databáze: OpenAIRE
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