Effects of Freezing and Supercooling on the I131Metabolism of Thyroid Tissue
| Autor: | Cavalieri Rr |
|---|---|
| Rok vydání: | 1963 |
| Předmět: |
medicine.medical_specialty
Autolysis (biology) Dimethyl sulfoxide medicine.medical_treatment Thyroid Thyroid Gland Metabolism Thyroglobulin Cold Temperature Guinea pig chemistry.chemical_compound Endocrinology medicine.anatomical_structure chemistry Iodine Isotopes Internal medicine Freezing medicine Humans Supercooling Incubation Iodine |
| Zdroj: | Endocrinology. 72:488-493 |
| ISSN: | 1945-7170 0013-7227 |
| DOI: | 10.1210/endo-72-3-488 |
| Popis: | Guinea pig thyroid lobes were frozen at –3 to – 5 C for 15 min, thawed, and incubated at 37 C with I131 iodide. Compared with control glands, kept at 2 C before incubation, there was less incorporation into thyroglobulin and a lower ratio of I131 DIT/MIT. Supercooling to – 8 C or freezing in the presence of dimethyl sulfoxide resulted in a normal pattern of iodination. The iodide-concentrating ability of pre-frozen tissue was only slightly lower than that of controls. Histologic studies indicated accelerated autolysis of colloid during incubation of pre-frozen tissue. The findings suggest that damage to specific cell structures by ice crystals leads to the observed defects in iodination. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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