Biodegradation of phenol by Chlamydomonas reinhardtii
Autor: | Theocharis T. Nazos, Demetrios F. Ghanotakis, Leonidas Mavroudakis, Spiros A. Pergantis |
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Rok vydání: | 2020 |
Předmět: |
0106 biological sciences
0301 basic medicine Bioenergetics Chlamydomonas reinhardtii Plant Science 01 natural sciences Biochemistry 03 medical and health sciences chemistry.chemical_compound Acetic acid Bioremediation Microalgae Phenol Catechol biology Chlamydomonas Cell Biology General Medicine Biodegradation biology.organism_classification Carbon Biodegradation Environmental 030104 developmental biology chemistry Energy Metabolism 010606 plant biology & botany |
Zdroj: | Photosynthesis Research. 144:383-395 |
ISSN: | 1573-5079 0166-8595 |
DOI: | 10.1007/s11120-020-00756-5 |
Popis: | The data presented in this particular study demonstrate that the biodegradation of phenol by Chlamydomonas reinhardtii is a dynamic bioenergetic process mainly affected by the production of catechol and the presence of a growth-promoting substrate in the culture medium. The study focused on the regulation of the bioenergetic equilibrium resulting from production of catechol after phenol oxidation. Catechol was identified by HPLC-UV and HPLC-ESI-MS/MS. Growth measurements revealed that phenol is a growth-limiting substrate for microalgal cultures. The Chlamydomonas cells proceed to phenol biodegradation because they require carbon reserves for maintenance of homeostasis. In the presence of acetic acid (a growth-promoting carbon source), the amount of catechol detected in the culture medium was negligible; apparently, acetic acid provides microalgae with sufficient energy reserves to further biodegrade catechol. It has been shown that when microalgae do not have sufficient energy reserves, a significant amount of catechol is released into the culture medium. Chlamydomonas reinhardtii acts as a versatile bioenergetic machine by regulating its metabolism under each particular set of growth conditions, in order to achieve an optimal balance between growth, homeostasis maintenance and biodegradation of phenol. The novel findings of this study reveal a paradigm showing how microalgal metabolic versatility can be used in the bioremediation of the environment and in potential large-scale applications. |
Databáze: | OpenAIRE |
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