Determination of rutin content in sophora japonia L. Extracts used as raw material in supplements by high performance liquid chromatography

Autor: Hong Hanh Nguyen Thi, Thu Dam Thi, Dat Nguyen Thanh, Thuy Chu Thi, Nuong Hoang Thi
Rok vydání: 2019
Předmět:
Zdroj: Heavy metals and arsenic concentrations in water, agricultural soil, and rice in Ngan Son district, Bac Kan province, Vietnam. 2:51-55
ISSN: 2615-9252
DOI: 10.47866/2615-9252/vjfc.80
Popis: An HPLC-UV method was proposed in order to determine Rutin in Sophora japonica L. extract samples (3 samples extracted with 30% ethanol, 3 samples extracted with 70% ethanol and 3 samples extracted with water). The chromatographic conditions were: Agilent Zorbax Eclipse XDB C18 Column (150 x 4.6 mm, 5 µm); UV-Vis detector (257 nm); mobile phase: a mixture of methanol and 1% acetic acid solution (40:60, v/v). The linear range was of 4.97 - 298.47µg/mL; the LOQ was of 0.205 µg/mL and the accuracy was within 99.87% - 102.3%. The method was proved to be suitable for the determination of Rutin in Sophora japonica L. extracts. Rutin content in samples varied from 30.14% to 38.67%.
Databáze: OpenAIRE