Autor: |
Hong Hanh Nguyen Thi, Thu Dam Thi, Dat Nguyen Thanh, Thuy Chu Thi, Nuong Hoang Thi |
Rok vydání: |
2019 |
Předmět: |
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Zdroj: |
Heavy metals and arsenic concentrations in water, agricultural soil, and rice in Ngan Son district, Bac Kan province, Vietnam. 2:51-55 |
ISSN: |
2615-9252 |
DOI: |
10.47866/2615-9252/vjfc.80 |
Popis: |
An HPLC-UV method was proposed in order to determine Rutin in Sophora japonica L. extract samples (3 samples extracted with 30% ethanol, 3 samples extracted with 70% ethanol and 3 samples extracted with water). The chromatographic conditions were: Agilent Zorbax Eclipse XDB C18 Column (150 x 4.6 mm, 5 µm); UV-Vis detector (257 nm); mobile phase: a mixture of methanol and 1% acetic acid solution (40:60, v/v). The linear range was of 4.97 - 298.47µg/mL; the LOQ was of 0.205 µg/mL and the accuracy was within 99.87% - 102.3%. The method was proved to be suitable for the determination of Rutin in Sophora japonica L. extracts. Rutin content in samples varied from 30.14% to 38.67%. |
Databáze: |
OpenAIRE |
Externí odkaz: |
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