Autor: |
Eun Hye Park, Hie-Joon Kim, Jin-Su Song |
Rok vydání: |
2012 |
Předmět: |
|
Zdroj: |
Bulletin of the Korean Chemical Society. 33:987-992 |
ISSN: |
0253-2964 |
DOI: |
10.5012/bkcs.2012.33.3.987 |
Popis: |
Magnetic nanoparticles (MNPs) treated with phosphoric acid were used to improve sequence coverage in protein identification by matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS). Sample solution of tryptic peptides from proteins was mixed with the MNPs, and the MNPs were separated from the supernatant using a magnet. MALDI mass spectra obtained separately from the supernatant and the MNPs were distinctly different and complementary to each other. Combination of the two spectra led to a significantly increased sequence coverage. |
Databáze: |
OpenAIRE |
Externí odkaz: |
|