Sequence Coverage Enhancement Using Magnetic Nanoparticles in Matrix-Assisted Laser Desorption/Ionization Mass Spectrometric Protein Analysis

Autor:	Eun Hye Park, Hie-Joon Kim, Jin-Su Song
Rok vydání:	2012
Předmět:	MALDI imaging Chromatography Chemistry Analytical chemistry General Chemistry Laser Spectral line law.invention chemistry.chemical_compound Matrix-assisted laser desorption/ionization law Desorption Mass spectrum Magnetic nanoparticles Phosphoric acid
Zdroj:	Bulletin of the Korean Chemical Society. 33:987-992
ISSN:	0253-2964
DOI:	10.5012/bkcs.2012.33.3.987
Popis:	Magnetic nanoparticles (MNPs) treated with phosphoric acid were used to improve sequence coverage in protein identification by matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS). Sample solution of tryptic peptides from proteins was mixed with the MNPs, and the MNPs were separated from the supernatant using a magnet. MALDI mass spectra obtained separately from the supernatant and the MNPs were distinctly different and complementary to each other. Combination of the two spectra led to a significantly increased sequence coverage.
Databáze:	OpenAIRE
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