Autor:	André M Comeau
Rok vydání:	2022
DOI:	10.17504/protocols.io.4r3l277k3g1y/v1
Popis:	The following detailed protocol is for the generation of paired-end sequencing reads of 16S/18S/ITS PCR amplicons with dual barcodes (i.e.: “indices”) on the Illumina MiSeq machine of length ≈450 bp (ie: 300+300 bp sequencing with ~150 bp overlap) using v3 600 cycle chemistry. It assumes an input of 380 individual samples (+4 controls = 384) in four 96-well plates. Original version of the protocol published in: https://journals.asm.org/doi/10.1128/mSystems.00127-16
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::b64a84cd073a909888cdfd21af936132 https://doi.org/10.17504/protocols.io.4r3l277k3g1y/v1 Zobrazit plný text záznamu