Preparing multiplexed 16S/18S/ITS amplicons for the Illumina MiSeq v1

Autor: André M Comeau
Rok vydání: 2022
Popis: The following detailed protocol is for the generation of paired-end sequencing reads of 16S/18S/ITS PCR amplicons with dual barcodes (i.e.: “indices”) on the Illumina MiSeq machine of length ≈450 bp (ie: 300+300 bp sequencing with ~150 bp overlap) using v3 600 cycle chemistry. It assumes an input of 380 individual samples (+4 controls = 384) in four 96-well plates. Original version of the protocol published in: https://journals.asm.org/doi/10.1128/mSystems.00127-16
Databáze: OpenAIRE