Preparing multiplexed 16S/18S/ITS amplicons for the Illumina MiSeq v1
Autor: | André M Comeau |
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Rok vydání: | 2022 |
Popis: | The following detailed protocol is for the generation of paired-end sequencing reads of 16S/18S/ITS PCR amplicons with dual barcodes (i.e.: “indices”) on the Illumina MiSeq machine of length ≈450 bp (ie: 300+300 bp sequencing with ~150 bp overlap) using v3 600 cycle chemistry. It assumes an input of 380 individual samples (+4 controls = 384) in four 96-well plates. Original version of the protocol published in: https://journals.asm.org/doi/10.1128/mSystems.00127-16 |
Databáze: | OpenAIRE |
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