Ein makroporöses Polyacrylamid für immunologische Diffusionstechniken / Macroporous Polyacrylamide, its Use for Immunological Diffusion Techniques
Autor: | Helmut Determann, Axel Walch |
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Rok vydání: | 1972 |
Předmět: | |
Zdroj: | Zeitschrift für Naturforschung B. 27:683-688 |
ISSN: | 1865-7117 0932-0776 |
DOI: | 10.1515/znb-1972-0617 |
Popis: | Gel layers of crosslinked polyacrylamide with different porosities were synthesized and their properties compared. At high concentrations of bifunctional monomers macroporous gels were formed, one of which (total monomer concentration 14%, methylene-bis-acrylamide 40%) permitted even high molecular weight proteins (e. g. γ-globulins) to diffuse more readily than in 2 per cent agar or agarose gels. Different immunological techniques were modified for application with this polymer. It offers advantages especially with respect to porosity, storability and sample application for quantitative diffusion techniques. |
Databáze: | OpenAIRE |
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