Ein makroporöses Polyacrylamid für immunologische Diffusionstechniken / Macroporous Polyacrylamide, its Use for Immunological Diffusion Techniques

Autor: Helmut Determann, Axel Walch
Rok vydání: 1972
Předmět:
Zdroj: Zeitschrift für Naturforschung B. 27:683-688
ISSN: 1865-7117
0932-0776
DOI: 10.1515/znb-1972-0617
Popis: Gel layers of crosslinked polyacrylamide with different porosities were synthesized and their properties compared. At high concentrations of bifunctional monomers macroporous gels were formed, one of which (total monomer concentration 14%, methylene-bis-acrylamide 40%) permitted even high molecular weight proteins (e. g. γ-globulins) to diffuse more readily than in 2 per cent agar or agarose gels. Different immunological techniques were modified for application with this polymer. It offers advantages especially with respect to porosity, storability and sample application for quantitative diffusion techniques.
Databáze: OpenAIRE