Purification of Anti-Steroid Antibodies for Use in Radioimmunoassay

Autor:	G. E. Abraham, W. D. Odell, D. R. Mishell, R. M. Nakamura, M. Labudovich
Rok vydání:	1972
Předmět:	Antiserum Chromatography biology Chemistry medicine.medical_treatment Biochemistry (medical) Clinical Biochemistry Fraction (chemistry) Radioimmunoassay Biochemistry Analytical Chemistry Steroid Titer Electrochemistry medicine biology.protein Stepwise elution Antibody Spectroscopy DEAE-Cellulose
Zdroj:	Analytical Letters. 5:105-113
ISSN:	1532-236X 0003-2719
DOI:	10.1080/00032717208066094
Popis:	An antiserum produced in a sheep against 11-desoxycortisol was fractionated using an ion-exchange column of DEAE cellulose. Using stepwise elution at fixed pH and increasing ionic strengths, three fractions were obtained. These fractions were tested for titer, affinity, and specificity. All three parameters studied showed differences between the various fractions. Fraction II showed a pattern similar to that of the whole antiserum. It is suggested that for optimization of radioimmunoassay, it may be advantageous to purify antisera in order to screen for immunoglobulin fractions with the best characteristics.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::a9cc0efba503947ddbf4d39254d38f23 https://doi.org/10.1080/00032717208066094 Zobrazit plný text záznamu