Fluorimetrische Bestimmung von intrazellulären Polyhydroxyalkanoaten
| Autor: | Simone Bieler, Matthias Noll, Alexander Kettner, Carola Griehl |
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| Rok vydání: | 2021 |
| Předmět: |
0303 health sciences
Chromatography biology 030306 microbiology Chemistry Pharmacology toxicology chemical and pharmacologic phenomena biology.organism_classification Fluorescence Polyhydroxyalkanoates 03 medical and health sciences Cytoplasm Bioreactor Fermentation Molecular Biology Intracellular Bacteria 030304 developmental biology Biotechnology |
| Zdroj: | BIOspektrum. 27:152-155 |
| ISSN: | 1868-6249 0947-0867 |
| DOI: | 10.1007/s12268-021-1558-8 |
| Popis: | Polyhydroxyalkanoates (PHA) are synthesized as intracellular carbon and energy storage compounds by many bacteria and are accumulated as granules in the cytoplasm of cells. Quantification of enriched PHA is of great importance for industrial production processes. Fluorescence measurements are particularly suitable for rapid determination of PHA during fermentation. With LipidGreen2 as fluorescent marker a fast and reliable assay for PHA monitoring in bioreactors was developed. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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