Development, optimization, and validation of magnetic solid-phase radioimmunoassay for estimation of luteinizing hormone

Autor:	S. M. Ayoub
Rok vydání:	2014
Předmět:	Matrix (chemical analysis) Chromatography Chemistry Solid phase radioimmunoassay Calibration Magnetic nanoparticles Radioimmunoassay Physical and Theoretical Chemistry Luteinizing hormone High-performance liquid chromatography Dilution
Zdroj:	Radiochemistry. 56:66-71
ISSN:	1608-3288 1066-3622
DOI:	10.1134/s1066362214010135
Popis:	The paper deals with the development, optimization, and validation of solid-phase radioimmunoassay (RIA) using magnetic particles for estimation of luteinizing hormone (LH) in human serum or plasma. 125I-LH was prepared by chloramine-T oxidation method and purified by HPLC. The activation of magnetic particles using 1,1′-carbonyldiimidazole (CDI) and coupling of these solid-phase particles with rabbit anti-LH were carried out. The assay was optimized and validated. The intra- and inter-assay variations were satisfactory. The recovery and dilution tests indicated accurate calibration and appropriate matrix. The present technique agreed well with currently used commercial kit (Siemens). These magnetic particles retain their characteristics during storage for 1 year at 4°C. The technique proved to be sensitive, specific, precise, and accurate for routine laboratory use.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::9dab338ecdd0facc2c0daac94b2896ec https://doi.org/10.1134/s1066362214010135 Zobrazit plný text záznamu Full text from SpringerLink