Spectroscopic determination of binding between human serum albumin and a platinum(II) dimethylsulfoxide complex
Autor: | Sh. A. Markaryan, K. R. Grigoryan, Mikayel Aznauryan, L. G. Gevorkyan, N. A. Bagramyan |
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Rok vydání: | 2008 |
Předmět: | |
Zdroj: | Journal of Applied Spectroscopy. 75:593-596 |
ISSN: | 1573-8647 0021-9037 |
DOI: | 10.1007/s10812-008-9070-1 |
Popis: | We have used electronic absorption and fluorescence spectroscopy to study binding between a platinum(II) dimethylsulfoxide complex (cis-[Pt(DMSO)2Cl2]) and human serum albumin (HSA), and the effect of complexation on the structure of the protein. We have calculated the binding parameters for binding between cis-[Pt(DMSO)2Cl2] and HSA. We have determined the binding constant KB = (1.2 ± 0.1)·103 M−1 and the Hill coefficient h = 1.03 ± 0.1. We have determined that binding between cis-[Pt(DMSO)2Cl2] and the protein leads to a change in the internal packing of the macromolecule. |
Databáze: | OpenAIRE |
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