Spectroscopic determination of binding between human serum albumin and a platinum(II) dimethylsulfoxide complex

Autor:	Sh. A. Markaryan, K. R. Grigoryan, Mikayel Aznauryan, L. G. Gevorkyan, N. A. Bagramyan
Rok vydání:	2008
Předmět:	Stereochemistry Chemistry chemistry.chemical_element Condensed Matter Physics Human serum albumin Binding constant Fluorescence spectroscopy Ultraviolet visible spectroscopy medicine Absorption (chemistry) Platinum Spectroscopy Macromolecule Nuclear chemistry medicine.drug
Zdroj:	Journal of Applied Spectroscopy. 75:593-596
ISSN:	1573-8647 0021-9037
DOI:	10.1007/s10812-008-9070-1
Popis:	We have used electronic absorption and fluorescence spectroscopy to study binding between a platinum(II) dimethylsulfoxide complex (cis-[Pt(DMSO)2Cl2]) and human serum albumin (HSA), and the effect of complexation on the structure of the protein. We have calculated the binding parameters for binding between cis-[Pt(DMSO)2Cl2] and HSA. We have determined the binding constant KB = (1.2 ± 0.1)·103 M−1 and the Hill coefficient h = 1.03 ± 0.1. We have determined that binding between cis-[Pt(DMSO)2Cl2] and the protein leads to a change in the internal packing of the macromolecule.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::8a225e46519d0208dd3e9918825e855c https://doi.org/10.1007/s10812-008-9070-1 Zobrazit plný text záznamu Plný text ve formátu PDF