Preparation and properties of thermo- and pH-responsive polypeptide bearing OEG and aldehyde pendants

Autor: Zuowen Shi, Fei Xu, Haoyu Tang, Ying Ling, Congping Zhou
Rok vydání: 2020
Předmět:
Zdroj: Colloid and Polymer Science. 298:1293-1302
ISSN: 1435-1536
0303-402X
DOI: 10.1007/s00396-020-04712-6
Popis: OEGylated polypeptide bearing triethylene glycol monomethylether (OEG3) and poly(ethylene glycol) methyl ether 350 (OEG7) were first prepared by copper-mediated 1,3-dipolar cycloaddition. 1H NMR, FTIR, and GPC results indicated the successful conjugation of oligo(ethylene glycol) (OEG) pendants with high grafting efficiency (≥ 98%). Variable temperature UV–vis spectroscopy revealed a reversible lower critical solution temperature (LCST)-type phase transition of the resulting polypeptides. The LCST-type cloud point temperature (Tcp) in the range of 20–60 °C can be readily tuned by changing the OEG3 and OEG7 composition. Thermo- and pH-responsive polypeptide was prepared by first conjugating of OEG and 6-aldehyde hexyl pendants, then crosslinking in the presence of 1,6-hexanediamine, yielding OEGylated polypeptide bearing pH-labile imine bonds. The resulting polypeptide showed pH-responsive property and less pronounced thermoresponsive property at high pH values (i.e., ≥ 6.15) and noticeable thermo- and pH- dual responsive properties at low pH values (i.e., ≤ 5.80).
Databáze: OpenAIRE
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