| Autor: |
Alberto Haces, Po Jen Shih, Babetta L. Marrone, Richard A. Keller, Mark Lewis Hammond, Charles W. Wilkerson, John D. Harding, Peter M. Goodwin, James H. Jett, John C. Martin, J.A. Schecker, W. Patrick Ambrose |
| Rok vydání: |
1993 |
| Předmět: |
|
| Zdroj: |
SPIE Proceedings. |
| ISSN: |
0277-786X |
| DOI: |
10.1117/12.146712 |
| Popis: |
We are developing a laser-based technique for the rapid sequencing of large DNA fragments (several kb in size) at a rate of 100 to 1000 bases per second. Our approach relies on fluorescent labeling of the bases in a single fragment of DNA, attachment of this labeled DNA fragment to a support, movement of the supported DNA into a flowing sample stream, sequential cleavage of the end nucleotide from the DNA fragment with an exonuclease, and detection of the individual fluorescently labeled bases by laser-induced fluorescence. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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