DNA sequencing by single molecule detection of labeled nucleotides sequentially cleaved from a single strand of DNA

Autor:	Alberto Haces, Po Jen Shih, Babetta L. Marrone, Richard A. Keller, Mark Lewis Hammond, Charles W. Wilkerson, John D. Harding, Peter M. Goodwin, James H. Jett, John C. Martin, J.A. Schecker, W. Patrick Ambrose
Rok vydání:	1993
Předmět:	DNA nanoball sequencing DNA clamp Biochemistry Oligonucleotide Base pair Chemistry GC-content DNA sequencing Sequencing by ligation Single molecule real time sequencing
Zdroj:	SPIE Proceedings.
ISSN:	0277-786X
Popis:	We are developing a laser-based technique for the rapid sequencing of large DNA fragments (several kb in size) at a rate of 100 to 1000 bases per second. Our approach relies on fluorescent labeling of the bases in a single fragment of DNA, attachment of this labeled DNA fragment to a support, movement of the supported DNA into a flowing sample stream, sequential cleavage of the end nucleotide from the DNA fragment with an exonuclease, and detection of the individual fluorescently labeled bases by laser-induced fluorescence.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::6b6adf06321021c634e7f2d657056415 https://doi.org/10.1117/12.146712 Zobrazit plný text záznamu