Graft-Versus-Host Disease Impairs Early B Lymphopoiesis in the Bone Marrow

Autor: Kristina Doser, Reinhard Andreesen, Martin Heidenreich, Petra Hoffmann, Tina J. Boeld, Matthias Edinger
Rok vydání: 2011
Předmět:
Zdroj: Blood. 118:2976-2976
ISSN: 1528-0020
0006-4971
DOI: 10.1182/blood.v118.21.2976.2976
Popis: Abstract 2976 Graft-versus-host disease (GVHD) and infectious complications are main causes of non-relapse mortality after allogeneic stem cell transplantation (SCT). Impaired immune function after SCT is usually attributed to the immunosuppressive medication applied for GVHD prophylaxis or therapy. Using a major histocompatibility complex (MHC)–mismatched murine model of GVHD (C57BL/6→BALB/c), we now examined the influence of GVHD on B cell immunity after SCT in the absence of pharmacologic immunosuppression. Lethally irradiated BALB/c (H-2d) recipients were transplanted with T cell-depleted bone marrow (TCD BM; 2.5×106) from C57BL/6 (H-2b) donors and parallel groups received CD4+CD25− conventional donor T cells (Tconv; 0.25 × 106) 2d later. Mice that received TCD BM alone (n =10) did not develop GVHD and showed a rapid and complete reconstitution of B cells in peripheral blood (PB) (25 ± 7% CD19+ B cells at d21; 55 ± 5% at d100). Mice that received additional donor Tconv cells (n =12) developed severe GVHD and completely lacked donor and host B cells in PB until their early death or throughout the observation period of 100d (p Disclosures: No relevant conflicts of interest to declare.
Databáze: OpenAIRE