Graft-Versus-Host Disease Impairs Early B Lymphopoiesis in the Bone Marrow
| Autor: | Kristina Doser, Reinhard Andreesen, Martin Heidenreich, Petra Hoffmann, Tina J. Boeld, Matthias Edinger |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
biology
business.industry Lymphocyte Immunology chemical and pharmacologic phenomena Cell Biology Hematology medicine.disease Biochemistry CD19 Transplantation surgical procedures operative medicine.anatomical_structure Graft-versus-host disease immune system diseases biology.protein Medicine Bone marrow Lymphopoiesis IL-2 receptor business B cell |
| Zdroj: | Blood. 118:2976-2976 |
| ISSN: | 1528-0020 0006-4971 |
| DOI: | 10.1182/blood.v118.21.2976.2976 |
| Popis: | Abstract 2976 Graft-versus-host disease (GVHD) and infectious complications are main causes of non-relapse mortality after allogeneic stem cell transplantation (SCT). Impaired immune function after SCT is usually attributed to the immunosuppressive medication applied for GVHD prophylaxis or therapy. Using a major histocompatibility complex (MHC)–mismatched murine model of GVHD (C57BL/6→BALB/c), we now examined the influence of GVHD on B cell immunity after SCT in the absence of pharmacologic immunosuppression. Lethally irradiated BALB/c (H-2d) recipients were transplanted with T cell-depleted bone marrow (TCD BM; 2.5×106) from C57BL/6 (H-2b) donors and parallel groups received CD4+CD25− conventional donor T cells (Tconv; 0.25 × 106) 2d later. Mice that received TCD BM alone (n =10) did not develop GVHD and showed a rapid and complete reconstitution of B cells in peripheral blood (PB) (25 ± 7% CD19+ B cells at d21; 55 ± 5% at d100). Mice that received additional donor Tconv cells (n =12) developed severe GVHD and completely lacked donor and host B cells in PB until their early death or throughout the observation period of 100d (p Disclosures: No relevant conflicts of interest to declare. |
| Databáze: | OpenAIRE |
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