Bi-functional labelling of DNA with tris(2-aminoethyl)amine-derived novel fluorescent agent

Autor: Kazuo Shinozuka, Yoshiaki Seto, Hiroyuki Kawata, Hiroaki Sawai
Rok vydání: 1993
Předmět:
Zdroj: Bioorganic & Medicinal Chemistry Letters. 3:2883-2886
ISSN: 0960-894X
DOI: 10.1016/s0960-894x(01)80784-8
Popis: A novel bi-functional fluorescent labelling agent derived from 5-hydroxy-1-naphthalene sulfonic acid and tris-2-aminoethyl)amine was synthesized and incorporated into the 5′-terminus of a 15-mer oligoDNA through phosphoroamidate linkage. The labelled oligoDNA bearing a primary and a tertiary amine group along with fluorescent naphthalene moiety exhibits improved hybridization ability with its complementary strand.
Databáze: OpenAIRE