Bi-functional labelling of DNA with tris(2-aminoethyl)amine-derived novel fluorescent agent
| Autor: | Kazuo Shinozuka, Yoshiaki Seto, Hiroyuki Kawata, Hiroaki Sawai |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
chemistry.chemical_classification
Tertiary amine Stereochemistry Organic Chemistry Clinical Biochemistry Pharmaceutical Science Tris(2-aminoethyl)amine Sulfonic acid Biochemistry Chemical synthesis Combinatorial chemistry Fluorescence chemistry.chemical_compound chemistry Labelling Drug Discovery Molecular Medicine Moiety Amine gas treating Molecular Biology |
| Zdroj: | Bioorganic & Medicinal Chemistry Letters. 3:2883-2886 |
| ISSN: | 0960-894X |
| DOI: | 10.1016/s0960-894x(01)80784-8 |
| Popis: | A novel bi-functional fluorescent labelling agent derived from 5-hydroxy-1-naphthalene sulfonic acid and tris-2-aminoethyl)amine was synthesized and incorporated into the 5′-terminus of a 15-mer oligoDNA through phosphoroamidate linkage. The labelled oligoDNA bearing a primary and a tertiary amine group along with fluorescent naphthalene moiety exhibits improved hybridization ability with its complementary strand. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|