| Autor: |
Siljak-Yakovlev, Sonja, Cerbach, Malika, Caulaud Joelle, Papeš, Dražena |
| Přispěvatelé: |
Huber, Đ. |
| Jazyk: |
angličtina |
| Rok vydání: |
1997 |
| Předmět: |
|
| Popis: |
A wild distributed species Picea abies and a paleoendemic (a tertiary relict) narrow localized Picea omorika have been cytogenetically investigated. Both of them showed identical chromosome number 2n=24, and morphologically similar karyotypes. We used some molecular cytogenetic techniques to find out whether any interspecific difference at chromosomal level exists. Two differential fluorescent banding techniques using A-T specific fluorescent dye Hoechst 33258 and G-C specific fluorescent dye Chromomycin A3, were applied as well as a fluorescent in situ hybridization method for detection of 18S and 5S rDNA gene clasters. The nuclear DNA content and the amount and relation between A-T and G-C base pairs were determined by flow cytometry technique. Obtained results showed a significant differentiation on chromosomal and genomic level which could explain the reproductive barriers between investigated Picea species. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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