Usporedna citogenetička istraživanja vrsta Picea abies (L.) Karsten i Picea omorika (Pančić) Purk

Autor: Siljak-Yakovlev, Sonja, Cerbach, Malika, Caulaud Joelle, Papeš, Dražena
Přispěvatelé: Huber, Đ.
Jazyk: angličtina
Rok vydání: 1997
Předmět:
Popis: A wild distributed species Picea abies and a paleoendemic (a tertiary relict) narrow localized Picea omorika have been cytogenetically investigated. Both of them showed identical chromosome number 2n=24, and morphologically similar karyotypes. We used some molecular cytogenetic techniques to find out whether any interspecific difference at chromosomal level exists. Two differential fluorescent banding techniques using A-T specific fluorescent dye Hoechst 33258 and G-C specific fluorescent dye Chromomycin A3, were applied as well as a fluorescent in situ hybridization method for detection of 18S and 5S rDNA gene clasters. The nuclear DNA content and the amount and relation between A-T and G-C base pairs were determined by flow cytometry technique. Obtained results showed a significant differentiation on chromosomal and genomic level which could explain the reproductive barriers between investigated Picea species.
Databáze: OpenAIRE