| Autor: |
FUKUMOTO, Mitsuki, KUDOU, Daizou, MURANO, Shouko, SHIBA, Tomoo, SATO, Dan, TAMURA, Takashi, HARADA, Shigeharu, INAGAKI, Kenji |
| Předmět: |
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| Zdroj: |
Bioscience, Biotechnology & Biochemistry; Jul2012, Vol. 76 Issue 7, p1275-1284, 10p |
| Abstrakt: |
The article analyses the role of active amino acid residues in the active sites of L-Methionine ɣ-lyase from Pseudomonas putida (MGL_Pp). It examines the properties of the amino acid Cystein 116 Histidine (C116H) mutant by kinetic analysis and three dimensional structural analysis. It reports that C116H mutant acquired a novel β elimination activity and lead a drastic conformation change in the histidine residue at position 116 by binding the substrate. |
| Databáze: |
Supplemental Index |
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