[Cultivation of murine B-cell hybridomas in the spleen].

Autor: Volgareva GM, Kil'diushevskaia TV, Del'vig AA, Kozhevnikova EV, Sviridov VV
Jazyk: ruština
Zdroj: Zhurnal mikrobiologii, epidemiologii i immunobiologii [Zh Mikrobiol Epidemiol Immunobiol] 1987 Jan (1), pp. 94-8.
Abstrakt: The tumorigenic capacity of mouse B-cell hybridomas in both cloned and primary cultures was studied. The cells were selected for inoculation from 24-well plates and introduced into the spleen of syngeneic mice. The cells took in 50% of the animals. The cells of hybridoma tumors induced as the result of intrasplenic inoculation, when subcultured in the second passage following the standard scheme, i.e. inoculated intraperitoneally in a dose of 1 X 10(7) cells into mice previously treated with vaseline oil or pristane, produced tumors in 100% of the animals.
Databáze: MEDLINE