Avaliação de várias concentrações de flúor na formação óssea ectópica em ratos jovens e velhos
| Autor: | Carvalho, Juliane Guimarães de |
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| Jazyk: | portugalština |
| Rok vydání: | 2005 |
| Předmět: | |
| Druh dokumentu: | Dissertação de Mestrado |
| Popis: | O presente trabalho avaliou as concentrações de 0 (controle), 5, 15 e 50 ppm de flúor na água na indução ectópica de osso em ratos jovens (90dias) e velhos (365 dias). Para tanto, 80 ratos Wistar, sendo 40 com 90 dias e 40 com 365 dias receberam implantes de matriz óssea alógena desmineralizada. Depois de 28 dias, os animais foram mortos e os implantes radiografados e analisados morfometricamente. Também foram obtidas amostras do fêmur e plasma dos animais, para análise de flúor com o eletrodo. A análise de flúor na superfície do fêmur foi feita pelo método direto e no fêmur e no plasma foi feita após difusão facilitada por HMDS. Os dados foram analisados por ANOVA e teste de Tukey, teste t e regressão linear (p This study evaluated the effects of various fluoride (F) concentrations (0, 5, 15 and 50 ppm) on ectopic bone formation in young and old rats. Eighty male Wistar rats were received as weanlings and were assigned to 4 groups (n=20/gr), which differed according to the F concentration they received in the drinking water. Groups 1, 2, 3 and 4 received water containing 0(control), 5, 15 and 50 mg F/L, respectively. When half of the rats were 90 days old, demineralized bone was implanted. The animals were killed 28 days after and implant, plasma and femur were collected. The other rats received demineralized bone matrix implants when they were 365 days old and were also killed 28 days after. The femur surface was analyzed for F with the ion-specific electrode. The femur ash and the plasma were analyzed for F with the ion specific electrode, following HMDSfacilitated diffusion. The implants were analysed by histomorphometry and radiography. Data were tested for statistically significant differences by ANOVA and Tukey´s test, t test and linear regression (p |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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