Erfassung und Bewertung der Immunzell-Dynamik ausgehend vom Transkriptom der Milz
| Autor: | Seth, Celina Marcella |
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| Jazyk: | němčina |
| Rok vydání: | 2024 |
| Předmět: | |
| Druh dokumentu: | Doctoral Thesis<br />Doctoral Thesis |
| DOI: | 10.25972/OPUS-37129 |
| Popis: | Erfassung und Bewertung der Immunzell-Dynamik ausgehend vom Transkriptom der Milz, Dissertation zum Vergleich von DCQ-Vorhersagen einzelner Immunzellpopulationen während akuter und chronischer LCMV-Infektionen mit experimentellen Daten. The outcome of viral infections is determined by a complex interaction between the spreading virus and the host's immune response. In order to understand this interaction in detail, acute and chronic infections were generated in previous experiments in the mouse model with the lymphocytic choriomeningitis virus and the transcriptomes of the entire spleen were determined in chronological order. This experimental approach results in the loss of information about the cells that produce the identified transcripts. This cell information can be recovered using special analysis programmes such as DCQ. However, it must then be confirmed using experimental methods. The aim of my work was to experimentally validate the prediction of individual immune cell populations. To this end, spleens were removed from LCMV-infected mice, the spleen cells were isolated and characterised by means of specific surface proteins using flow cytometry. I was thus able to partially validate the DCQ predictions of different immune cells and immune cell subtypes such as CD8+ T cells, CD103+ DCs, neutrophils, regulatory CD4+/ FOXP3+ T cells, NK cells and monocytes. These results made it possible to correlate the dynamics of the different cell types with immunological processes. The results were included in a previously published paper (Pedragosa et al., 2019). |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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