| Popis: |
This thesis consists of three published articles and one submitted article, and is focused on the evaluation of the effects of vegetation on soil microbial communities and processes on brown coal post-mining sites. The research sites served as deposits of infertile waste material, by which the indigenous vegetation has been buried and thus the whole ecosystem was destroyed. The restoration of the ecosystem structure and functions at these areas was based on the improvement of biotic and abiotic properties of excavated soil substrate either using assisted afforestation or using spontaneous plant succession. In this work, biochemical and molecular techniques were employed in order to testify the effect of the vegetation on soil microbial processes, structure of soil microbial communities and to follow the chemical changes in the litter composition during its decomposition. Litter chemistry, enzymatic activities and microbial biomass were followed by standard biochemical procedures including spectrometry and HPLC. Bacterial microarray was used for assessing the composition of bacterial communities in the soil of successional plots. 454-pyrosequencing was employed for detailed identification of fungal and bacterial community composition in the litter layer and the bulk soil under different tree... |
