| Popis: |
Background: Atopic dermatitis (AD) is a chronic inflammatory skin disease with acute exacerbations and characteristic clinical manifestations. The diagnosis of AD is based on the medical history, evaluation of clinical condition, supplemented by skin or exposure tests. An integral part is also the examination of specific IgE antibodies against extract allergen or single molecular component. Biochip multiplexing techniques have recently been used for respiratory forms of allergic inflammation and when food allergy is suspected. However, these analyses could find application in the diagnosis of cutaneous manifestations of allergic inflammation, such as AD. The aim of this work was to more acurately identify the source of food and inhalant allergy in patients with AD. We evaluated sensitization profiles in patients with mild, moderate, and severe forms of AD and in a subset of patients with AD complicated by bronchial asthma and allergic rhinitis. We also addressed the sensitization to molecular components in the context of food allergy in patients with AD. Methods: Blood serum samples were taken from adult patients with AD. Levels of specific IgE antibodies were determined by the ImmunoCAP ISAC multiplex system. The dissertation thesis summarises already published results of our scientific team... |
