| Popis: |
The aim of this thesis was to observe how polyunsaturated fatty acids affect the wound healing process. This effect was observed by expression of selected genes and collagen type I and III in the model organism. The Wistar Albino laboratory rat strain was chosen as a model organism. 50 rats were used for the experiment. Rats were divi-ded into five groups of ten. One control group was selected which was fed only with compound feed without the addition of oil. The rats in the remaining groups were fed a feed mixture with the addition of oil (Schizochytrium extract oil, fish oil, palm oil and safflower oil). After 52 days of fattening, rats under anesthesia were excised on the dor-sal side of the back. Fattening was continued for 12 days and then rats were sacrificed with isoflurane to remove liver and healing skin wounds. EPA / DHA deposition was determined from collected liver. RNA was obtained from healing skin wounds for quan-titative PCR with specific primers for TGF-β1, PTGS2, ACTA2, VEGF, COL1A1, COL3A1. Further, immunohistochemical sections with type I and III collagen monoclo-nal antibodies were generated from the healing skin wound. The assumption of achie-ving better results with high DHA oil was not lost. Best results in the wound healing process were achieved in rats fed with safflower and fish oil (p |
