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This master‘s thesis deals with ecotoxicity assessment of plasma-activated solutions. Various plasma-activated solutions were prepared in the experiments. In the vast majority of tests, it was plasma-activated tap water – PAW. Depending on the test organism, dilution water was further processed, namely dilution water for Thamnocephalus platyurus and Daphnia magna. In this master‘s thesis the ecotoxicological effects were assessed through aquatic ecotoxicity tests with an aquatic plant Lemna minor, on a crustaceans T. platyurus and D. magna and on agricultural crops Lactuca sativa and Allium cepa L.. Crustaceans are fresh-water organisms on which the water contamination can be quickly and quite easily assessed. These plasma-activated solutions were also tested for acute ecotoxicity of soil organisms by ecotoxicological contact tests with Eisenia fetida, springtails Folsomia candida and seeds of L. sativa. Tests on T. platyurus and D. magna were performed in time intervals and a change in mortality was observed depending on PAW concentration. If the test results allowed, the LC50, IC50, A and NR escapism values were calculated. When the measured and calculated values did not allow such a calculation, the overall effect of PAW on organisms was assessed and a significant difference was calculated for the L.sativa root growth inhibition test. The final results showed that PAW has an ecotoxic effect on aquatic organisms, while it has rather beneficial effects on terrestrial organisms. |
