| Popis: |
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Title, Name, Surname of candidate: Klára Štěrbová Title, Name, Surname of tutor: Doc. RNDr. Lenka Skálová, Ph.D. Title of a diploma work: Induction and inhibition of acenaphthenol dehydrogenase Breast cancer belongs to hormone-dependent tumours. Apart from lifetime exposure to estrogens, the volume of female active sex hormones in breast tissue also plays a big part in the development of this disease. One of mechanisms regulating concentration of estrogens in the target tissue at the pre-receptor level is their interconversion from inactive estron to its active form, 17β-estradiol enzymes from the aldo-ketoreduktas 1C (AKR1C) subfamily. On the basis of specific substrates, inductive and inhibitive studies, enzymes involved in the conversion of individual hormones can be characterized. Relatively specific substrate for AKR1C is acenaphthenol (AcNOH). However, due to the fact that in a multi-enzyme model system the involvement of other enzymes in the reaction cannot be excluded, the enzymes which take part in conversion of acenaphthenol labeled "acenaphtenol dehydrogenase" (AND). The Aim of my diploma paper is to spectrofluorimetrically determine the activity of AND in cytosole taken from breast... |
