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Candidate: Markéta Horáčková Title of diploma thesis: Taxonomic characterization of new Halorubrum isolates from hypersaline soils. Charles University, Faculty of Pharmacy in Hradec Králové Department of Biological and Medicinal Sciences University of Sevilla, Faculty of Pharmacy Department of Microbiology and Parasitology Study program: Pharmacy The aim of this work is to continue in taxonomic characterization of new strains of the genus Halorubrum isolated from hypersaline soils from Paraje Natural Marismas del Odiel, Huelva, Spain. These Archaea are micro-organisms that require high salt concentration in their environment. With the characterization started Ana Durán Viseras last year and I participated on continuation of this characterization. To complete phylogenetic study we performed MLSA of rpoB gene of each sample to create phylogenetic trees. Based on results of the phylogenetic study we inferred that studied strains could constitute new species. We determined the guanine plus cytosine content in DNA and also carried out phenotypic studies as important part of the taxonomic characterization of the new species. |
