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in English Vertebrates host complex microbial communities in their intestinal system. This gut microbiome (GM) comprises billions of bacterial cells closely interacting with a host's physiology. Despite contemporary progress in this field, present knowledge of vertebrate GM is based on studies carried out only on a limited set of host species, including humans, captive rodents and few economically important mammals and birds. On the contrary, knowledge of GM in wild living, non-mammalian species is still rather insufficient. Thus, this thesis aims to analyze GM in wild passerine populations and to investigate potential patterns in GM composition. Main objective is to provide evidence that GM composition is dependent on host species, and of co-divergence between GM composition variability and host phylogeny. Additional focus is devoted to diversity analysis among individual bacterial genera regarding their phylogeny. Using the Illumina MySeq platform, we sequenced amplicons of bacterial 16s rRNA gene obtained from fecal samples of 486 individuals representing 57 Czech Republic passerine species and 107 individuals representing 38 Cameroon Republic species. A modified approach was applied during data processing. In the first step, analyzed dataset was sorted by classifying sequences to respective... |
