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Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Michaela Žaludová Supervisor: Dr. Maria Albertovna Khalikova, Ph.D. Consultant: doc. RNDr. Dalibor Šatinský, Ph.D. Title of Diploma Thesis: Determination of illegal compounds in sport supplements by modern chromatographic methods This diploma thesis concerns on the development of method for the determination of steroid hormones in sport supplements using ultra-high performance supercritical fluid chromatography. The method development and optimization of chromatographic conditions include selection of a suitable stationary phase and a modifier together with a mobile phase additive. Furthermore, the influence of temperature, pressure, flow rates and mobile phase gradient on separation efficiency was examined. In conclusion, this method has been validated and applied on real samples. Experiments were carried out on the device ACQUITY UPC2 with PDA detector. Detection was at wavelength 232 nm. 11 compounds were analysed. The stationary phases screening included 5 different analytical columns ACQUITY UPC2 BEH 1,7 µm, BEH 2-EP 1,7 µm, HSS C18 1,7 µm, CSH Fluoro-Phenyl 1,7 µm and 2-PIC 1,7 µm dimensions 3,0 x 100 mm. As the most suitable was selected column HSS C18 1,7 µm (3,0 x 100 mm)... |
