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Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Botany and Ecology Candidate: Lucie Křížková Supervisor: RNDr. Jitka Vytlačilová, Ph.D. Title of diploma thesis: Ecotoxicological study of fluoroquinolone antibiotics mixture This thesis is focused on ecotoxicological evaluation of the risk of two fluoroquinolone antibiotics - ciprofloxacin and norfloxacin. A multigenerational test with a protozoan Tetrahymena thermophila, which is based on the model of microbiotest of Protoxkit F, has been used for their evaluation. The inhibitory effect of the tested substances on growth and reproduction of organisms has been observed. The dependence between inhibitory effect and concentration of the pharmaceuticals had been detected, and than the values of 24h EC50 have been calculated. During the tests, the phototoxic potential of both antibiotics has been observed and also the mixtures of ciprofloxacin and norfloxacin (1:1, 1:2, 2:1) have been tested. Keywords: ecotoxicity, ciprofloxacin, norfloxacin, Tetrahymena thermophila, phototoxicity |
