Fylogeneze krvetvorby obratlovců
| Autor: | Svoboda, Ondřej |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
progenitory
megakaryocyty scf erythrocytes progenitors trombocyty CFU-TE paralog differentiation chicken granulocyty HSC sebeobnova dánio thrombocytes krvetvorba gcsf cytokines zebrafish TEP colonies diferenciace clonal assays megakaryocytes klonální kultura CFU-E kuře cytokiny kolonie homolog CFU-T ZEB hematopoiesis WKM tpo erytrocyty epo MEP BFU-E granulocytes self-renewal |
| Druh dokumentu: | Doctoral Thesis |
| Popis: | (ENGLISH) Hematopoiesis is dependent on the actions of hematopoietic stem cells (HSCs). This process is tightly controlled through a complex array of extrinsic and intrinsic factors. Even though the hematopoiesis seems to be well conserved across the disparate vertebrate animals, erythroid and thrombocytic differentiation have changed during the evolution of mammals. Specifically, adult mammalian red blood cells have the unique feature of being enucleated, and mammalian thrombocytes are not individual cells, but fragments of megakaryocytes, instead. It is likely that these enhancements provided a survival advantage to early mammalian species; however, they also bring up the question of evolutionary origin of these cells that studied using zebrafish (Danio rerio) model. First, it was necessary to generate a toolbox of a recombinant cytokines and optimized culture media that allowed us to manipulate zebrafish hematopoietic cells ex vivo in liquid and clonal cultures. Interestingly, teleost species underwent an extra duplication event during their evolution and as a result, two copies (paralogs) of some of the genes are present in zebrafish. This was also the case for majority of the cytokines from our toolbox and here, we provide functional characterization of these paralogs. Strikingly, our results... |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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