| Popis: |
It is known, that grape seeds are rich in significant antioxidant. The issue of dealing with the analysis and comparison of antioxidant components in the seeds of Vitis vinifera in the individual varieties has not been sufficiently studied, yet. In the first part of the thesis is an extensive literature focused on a summary of the work, which study substances in the vine seeds and their effect on human health. Experimental work is focused on the study of antioxidant components in the seeds of Vitis vinifera. The experiment was used extracts of the three "normal" (Frankova, Ryzlink vlašský and Cabernet Moravia) and three interspecific varieties (Nativa, Marlen and Kofranka). Spectrometric, using three fundamentally different methods - DPPH, ABTS and FRAP was determined antioxidant activity. Spectrophotometry was further investigated the content of total polyphenolic compounds and total content of flavanols. Technique of high- performance liquid chromatography with mass spectroscopy were analyzed nine major flavonoids (apigenin, astragalin, hyperoside, isorhamnetin, kaempferol, myricetin, quercetin, quercitrin and rutin) and two procyanidins (procyanidin A2 and procyanidin B1). The highest values of antioxidant reached interspecific varieties Marlen and Nativa, at the least antioxidant components were recorded in the variety Cabernet Moravia. However, in complex evaluation, between studied of varieties were not significant differences. |
