Non-targeted Effects of lonizing Radiation in Fish Cell Lines

Autor: O'Neill, Alicia
Jazyk: angličtina
Rok vydání: 2006
Předmět:
Popis: This study is one of the first to examine non-targeted effects of radiation in fish cell lines, with the aim of identifying a reliable reporter system for evaluating radiation damage in fish. The ability of the fish cell lines to clone was determined as the clonogenic assay was a major end point used to measure survival. A direct survival curve was completed for all cell lines that were deemed clonogenic using a cobalt-60 γ-radiation source. Non-targeted effects of radiation were evaluated by conducting bystander experiments on all fish cell lines. Delayed Cell Death (DCD) experiments were completed on the fish cell line that showed evidence of a cell death associated bystander effect as these phenomena may be linked. Four of the eight cell lines were found to be clonogenic. The cell line, RTG-2, was found to be the most radiosensitive at lower doses. All of the clonogenic cell lines, with the exception ofRTG-2 cells, generally showed increased Plating Efficiency (P.E.) when Irradiated Cell Conditioned Media (ICCM) was tested on unirradiated autologous cells. ICCM from the clonogenic and non-clonogenic cells was also tested on the mammalian cell line. This resulted in increased cell survival, with the exception of the RTS-pBk+ (p
Thesis
Master of Science (MSc)
Databáze: Networked Digital Library of Theses & Dissertations