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Dielectrophoresis (DEP) is the induced motion of polarizable particles in non- uniform electric fields. Used for many years for the manipulation of particles from cell-scale to macromolecules. This thesis presents the application of the technique for manipulation of DNA, carbon nanotubes and nanowires properties. The DNA containing only adenine-thymine (poly AT) bases, and containing only cytosine- guanine (poly GC) were analysed using microfabricated electrode structures. Poly AT was stained with DAPI and JOJO-l for poly GC DNA. It was found that there were differences between the frequency-dependent DEP behavior of the two molecules. When looking at the difference between the two types on crossover frequency (the point where DEP changes from attractive to repulsive), it was found that they varied by up to a factor of 2. This point to possible insights in the charge conduction mechanism in different DNA forms, as well as potential new mechanisms for gene separations and sequencing. Moreover, the research of DEP on nanowires has been carried out to implement a novel application of nanomanipulation. Organic and inorganic nanomaterial have been studied intensively using ionic liquids as their medium of conductivity. A DEP impedance measurement of the single-walled carbon nanotubes, zinc oxide (nano powder) and tin oxide (nano powder) provides a technique for automated collection for nanowires. The effects ofDEP manipulation on the Bovine Serum Albumin has been successfully demonstrated as a viable technique to assemble nano-sized objects. |
