| Autor: |
Sainsbury, Frank |
| Rok vydání: |
2008 |
| Předmět: |
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| Druh dokumentu: |
Electronic Thesis or Dissertation |
| Popis: |
Expression vectors based on the bipartite Cowpea Mosaic Virus (CPMV) have been investigated for use in molecular farming in plants. Agroinfiltration into Nicotiana benthamiana of two full-length RNA-2 constructs containing different marker genes in the presence of RNA-1 showed that two foreign proteins can be efficiently expressed within the same cell in inoculated tissue. However, segregation of the RNA-2 molecules was observed upon systemic spread of the recombinant viruses. Thus efficient assembly of heteromeric proteins is likely to occur only in inoculated tissue. Therefore vectors based on full-length RNA-2 molecules do not have an advantage over vectors based on deleted versions of RNA-2 that are restricted to infiltrated tissue. |
| Databáze: |
Networked Digital Library of Theses & Dissertations |
| Externí odkaz: |
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