Proteomics tools reveal startlingly high amounts of oxytocin in plasma and serum
| Autor: | Brandtzaeg, Ole Kristian, Johnsen, Elin, Roberg-Larsen, Hanne, Seip, Knut Fredrik, MacLean, Evan L., Gesquiere, Laurence R., Leknes, Siri, Lundanes, Elsa, Wilson, Steven Ray |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Druh dokumentu: | Článek |
| ISSN: | 2045-2322 27528413 |
| DOI: | 10.1038/srep31693 |
| Popis: | The neuropeptide oxytocin (OT) is associated with a plethora of social behaviors, and is a key topic at the intersection of psychology and biology. However, tools for measuring OT are still not fully developed. We describe a robust nano liquid chromatography-mass spectrometry (nanoLC-MS) platform for measuring the total amount of OT in human plasma/serum. OT binds strongly to plasma proteins, but a reduction/alkylation (R/A) procedure breaks this bond, enabling ample detection of total OT. The method (R/A + robust nanoLC-MS) was used to determine total OT plasma/serum levels to startlingly high concentrations (high pg/mL-ng/mL). Similar results were obtained when combining R/A and ELISA. Compared to measuring free OT, measuring total OT can have advantages in e.g. biomarker studies. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
| Externí odkaz: |
|