Identificação e caracterização molecular das lacases de eucalipto (Eucalyptus grandis)
| Autor: | Arcuri, Mariana de Lara Campos. |
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| Jazyk: | portugalština |
| Rok vydání: | 2017 |
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| Druh dokumentu: | Computer File |
| Popis: | Orientador: Ivan de Godoy Maia Laccases are p-diphenol-O2-oxidoreductases encoded by multigene families widely distributed throughout the plant kingdom. They exhibit important roles in the oxidation of monolignols during lignin biosynthesis and are reported to be functionally involved in plant development, tolerance and response to stress. Apart from plants, laccases can be also found in bacteria, fungi and insects. Here, a genome-wide survey of the eucalyptus genome revealed the presence of 88 putative laccases genes. However, after meticulous analyzes using different approaches, the redundant sequences were discarded and 54 laccases genes (referred as EgLAC) were retrieved. These genes were phylogenetically distributed in six different subgroups. Based on RNA-Seq data, distinct organ/tissue expression patterns of the identified EgLAC genes were ascertained. The vast majority showed organ/tissue-enriched expression, while certain genes exhibited no detectable expression. RT-qPCR analyzes confirmed the organ/tissue expression patterns of a representative set of genes such as, for example, the rootspecific expression of EgLAC52 and the root and leaf preferential expressions of genes EgLAC4 and EgLAC32. Further expression profiling of selected EgLAC genes in response to oxidative and osmotic stresses revealed differences in their relative expression, with some genes being stress-induced. These results suggest that certain laccases might be implicated in abiotic stress responses. Mestre |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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