Nuclear Strain Attenuation in Wavy Cells
| Autor: | ON-NEI WU, 胡安妮 |
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| Rok vydání: | 2018 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 107 In many collagen-rich tissues, such as vascular wall, ligament, and tendons, the fibrillary extracellular matrix is organized in a wavy structure, known as crimp. The embedded cells and their nuclei follow this structure and have a wavy morphology. Studies have shown that chromatin organization and some gene expression might be regulated by nuclear morphology. We are interested in how cell geometry regulate nuclear response upon stretch. When mesenchymal stem cells (MSCs) were stretched on wavy electrospun fibers, we observed that the cell was straightened rather than elongated. In order to have better control of cell morphology, MSCs were seeded on PDMS membranes microcontact-printed with fibronectin lines and examined real-time on a stretching bioreactor. Intranuclear Lagrangian strain field revealed that wavy cell geometry caused significant nuclear strain attenuation and this phenomenon is regulated by myosin. In addition, zyxin was exported form nuclei in wavy cells whereas the opposite trend was observed in straight cells. Our results indicated that cell morphology regulate stretch-induced nuclear mechanotransduction. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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