Particles and hydrogels manipulation to construct hepatic lobule tissue on electromicrofluidic platform
| Autor: | Kuan-Lun Ho, 何寬倫 |
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| Rok vydání: | 2018 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 107 In this study, electromicrofluidic (EMF) platform integrating electrowetting-on-dielectric (EWOD) and dielectrophoresis (DEP) forces is used to pattern multiple hydrogel prepolymers containing solid particles into in-vivo-like hepatic lobule arrangements. Biocompatible hydrogel with different concentration and stiffness was manipulated by the electromicrofluidic platform to construct a hydrogel-based scaffold for cell culture and form an in vitro liver tissue with proper cell morphology and phenotypic function, and our goal is to engineer a liver lobule tissue with blood vessel formation. Therefore, four kinds of hydrogels were used in this research, including photo-crosslinkable hydrogel, gelatin methacryloyl (GelMA) and polyethylene glycol diacrylate (PEGDA), chemically-crosslinkable hydrogel, fibrin gel, and thermally-crosslinkable hydrogel, matrigel. On the other hand, human hepatocellular carcinoma (Huh-7), human umbilical vein endothelial cells (HUVEC) and differentiated human monocytic cells, THP-1 were cultured in biocompatible hydrogels to form the in-vitro biomimetic liver tissue. To construct in vitro hepatic-lobule-like microstructures, Huh-7 and HUVEC mixed with THP-1 were dispersed in GelMA prepolymers respectively with appropriate concentrations. Furthermore, we measured the albumin secretion from different culture conditions and evaluated the function of our liver lobule tissue. For the applications, we investigated the influence of drug hepatotoxicity on liver function. At last, to improve the cell proliferation of HUVEC, we have cultured HUVEC in different extracellular matrixes, expecting that the microvascular formation of endothelial cells may improve the function of our liver tissue. In the future, this engineered tissue can serve as an ideal in vitro model for basic medical research, drug screening and pathogen interaction. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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