The influence of halide promoters and acceptors on glycosylation and the real-time analysis by thin-layer chromatography for solid-phase reaction.
| Autor: | Chia-Hui Wu, 吳佳蕙 |
|---|---|
| Rok vydání: | 2018 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 107 We have discussed the roles of the intermediates, glycosyl halide and glycosyl triflate, during the glycosylation and proposed a plausible mechanism for p-TolSCl/AgOTf and NXS/TfOH conditions. Additionally, the reactivity of acceptor influences the stereoselectivity; namely, higher reactive acceptor prefers β-glycosylation and lower reactive acceptor favors α-product. The DMF-assisted stereoglycosylation was utilized in the solid-phase glycosylation successfully. Also, the real-time detection by thin-layer chromatography (TLC) for the solid-phase reaction was investigated and is believed to benefit this field. Finally, we functionalized and fucosylated a nonasaccharide based on the research in this thesis. The obtained nonasaccharide is going to further study. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
| Externí odkaz: |
|